000			03215cam a2200349 i 4500
001			17750397
003			OSt
005			20161227164534.0
008			130523t20142014nyua bf 001 0 eng
010			_a 2013019474
020			_a1936113813 (paper)
040			_aDLC _beng _cDLC _erda _dDLC
042			_apcc
082	0	0	_a571.9 _223
245	1	0	_aAntibodies : _ba laboratory manual / _cedited by Edward A. Greenfield, Dana-Farber Cancer Institute.
250			_aSecond edition.
300			_axxi, 847 pages : _billustrations ; _c29 cm
520			_a"The focus of Antibodies: A Laboratory Manual, 2nd Edition, will be unchanged from the original edition by Ed Harlow and David Lane and will cover both the production and use of antibodies in a way that is accessible to the nonimmunologist. The emphasis will be on contemporary, essential antibody-based methods that are tried, true, necessary, and useful to a broad population of life scientists. The manual will provide up-to-date protocols that work reproducibly, along with explanations as to how and why methods work and how to choose between alternative approaches. Methods that have become research staples since the manual was originally published will be included at the same level of detail and organization as the existing topics"-- _cProvided by publisher.
650		0	_aImmunoglobulins _vLaboratory manuals. _99841
650		0	_aImmunochemistry _vLaboratory manuals. _99842
650		7	_aSCIENCE / Biotechnology. _2bisacsh _99843
650		7	_aSCIENCE / Life Sciences / Biochemistry. _2bisacsh _99844
650		7	_aSCIENCE / Life Sciences / Biology / Molecular Biology. _2bisacsh _99845
700	1		_aGreenfield, Edward A., _d1957- _98197
130	0		_aUsing antibodies. _99846
264		4	_c©2014
337			_aunmediated _2rdamedia
338			_avolume _2rdacarrier
504			_aIncludes bibliographical references and index.
505	8		_aMachine generated contents note: -- Preface -- 1 Antibody Production by the Immune System -- Stefanie Sarantopoulos -- 2 The Antibody Molecule -- Stefanie Sarantopoulos -- 3 Antibody-Antigen Interactions -- Stefanie Sarantopoulos -- 4 Antibody Responses -- Stefanie Sarantopoulos -- 5 Selecting the Antigen -- Edward A. Greenfield, James DeCaprio, and Mohan Brahmandam -- 6 Immunizing Animals -- Edward A. Greenfield -- 7 Generating Monoclonal Antibodies -- Edward A. Greenfield -- 8 Growing Hybridomas -- Edward A. Greenfield -- 9 Characterizing Antibodies -- Frances Weis-Garcia and Robert H. Carnahan -- 10 Antibody Purification and Storage -- Jordan B. Fishman and Eric A. Berg -- 11 Engineering Antibodies -- James Dasch and Amy Dasch -- 12 Labeling Antibodies -- Eric A. Berg and Jordan B. Fishman -- 13 Immunoblotting -- Larisa Litovchick -- 14 Immunoprecipiation -- James DeCaprio and Thomas O. Kohl -- 15 Immunoassays -- Thomas O. Kohl and Carl A. Ascoli -- 16 Cell Staining -- Scott J. Rodig -- 17 Antibody Screening using High Throughput Flow Cytometry -- Thomas D.l. Duensing and Susan R. Watson -- Appendix I: Electrophoresis -- Appendix II: Protein Techniques -- Appendix III: General Information -- Appendix IV: Bacterial Expression -- Appendix V: Cautions -- Index.
942			_2ddc _cBK
999			_c70912 _d70912